Akta Pure 25 Manual

AKTA pure 25 New Owner?s User Guide . The exercise below will give a quick demonstration of how easy and intuitive the AKTA pure 25 will be for you in demonstrating downstream processing to your students. Steps include: ? Opening the Unicorn software windows for manual control and programmable features ? Purging/priming pumps and tubing, ŽKTA? pure UserManual. TableofContents 1 Introduction ... 2.2Liquidflowpath 25 2.3Instrumentcontrolpanel 27 2.4Instrumentmodules 32 2.5Installationofinternalmodules 76 2.6Accessories 79 ŽKTApureUserManual29119969AC 13 2 TheŽKTApureinstrument. 2.1 Overviewillustrations Introduction, AKTA pure 25 New Owner?s Intro The exercise below will give a quick demonstration of how easy and intuitive the AKTA pure 25 will be for you in demonstrating downstream processing to your students. Steps include: Opening the Unicorn software windows for manual control and programmable features Purging/priming pumps and tubing, version ŽKTA? pure 25 (1.10.0.6) ?Peak Collect tubing kit to direct peak to subsequent steps, product code 29314678 ?Method Queue functionality to run capture and polishing steps without manual interactions during run Automatic transfer of target protein (peak) and start of second method, 1 V9-Inj Col Column inlet 0. 25 220 2 Column outlet U9-M inlet 0. 25 60 The different PEEK tubing used in ŽKTA pure has different colors: ? id 0.15 mm violet ? id 0. 25 mm blue ? (id 0.5 mm orange, id 0.75 mm green, though not used in this Cue Card) 3 Setup for SEC analysis 3.1 System configuration from injection valve to UV-monitor without.

AKTA pure 25 New Owner?s Intro - Biomanufacturing, ŽKTA pure | Cytiva, ŽKTA pure - Cytiva, AKTA pure 25 New Owner?s Intro - Biomanufacturing, For ŽKTA? pure 25 and ŽKTA? pure micro, the system pumps provide a flow rate range of up to 25 mL/min at maximum operating pressure of 200 bar (2900 psi, 20 MPa). For ŽKTA? pure 150 the flow rate is up to 150 mL/min at maximum operating pressure of 50 bar (725 psi, 5 MPa). For column packing, ŽKTA? pure 25, Manual control of the instrument 3.5. Equilibration of system and column 3.6. Fraction collector setup ... AKTA pure 25 Users Guide (electronic) 4.3. Manufacturer?s literature/spec sheets for media ... For the Unicorn 6.3 software to be able to control the AKTA pure instrument, it is necessary to assemble programmed steps into a method. The.

ŽKTA avant 25 0.6, 1.4, and 5 ml ŽKTA avant 150 1.4, 5, and 15 ml Fig 3. For increased operational safety, the column selection valve enables continuous measurement of precolumn (Pre-CP) and post-column pressure (Post-CP) during runs. The pressure difference over the packed medium bed (?p) is calculated from the two signals. System pump pressure, With a low hold-up volume flow path, the ŽKTA pure micro system is your solution for small sample volumes and micropreparative columns. Combined with Fraction collector F9-T, the system can help you purify proteins when sample volumes are low ? for example, when preparing for cryo-EM experiments.If you already have an ŽKTA pure 25 M system in your lab, you can use the Micro kit to convert.

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