The chrome azurol S (CAS) assay is a universal colorimetric method that detects siderophores independent of their structure. In this assay, siderophores scavenge iron from an Fe-CAS-hexadecyltrimethylammonium bromide complex, and subsequent release of the CAS dye results in a color change from blue to orange.
Chromeazurol S CAS Number: 1667-99-8 EC Number: 216-787-0 Synonyms: Mordant Blue 29, Chrome Azurol S find Sigma-Aldrich -C1018 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich, Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Alexander. Biology and Fertility of Soils, 12, 39-45 (1991) Phosphate Solubilizing Rhizobacteria Could Have a Stronger Influence on Wheat Root Traits and Aboveground Physiology Than Rhizosphere P.
Siderophore Detection Using Chrome Azurol S and Cross.
Siderophore Detection Using Chrome Azurol S and Cross.
Polymer producing bacteria showing siderophore activity with.
Chrome Azurol S (CAS) Assays for Iron-Binding Compounds, using chrome azurol S (CAS) and hexadecyltrimethylammonium bromide (HDTMA) as indicators. chrome azurol S (CAS) agar effectively differentiated bacteria that are capable of exereting large amounts of siderophore. In the current investigation, we focused on performing siderophore activity for the all 12 isolates, the center of a Chrome Azurol S agar ((compositions are 60.5 mg of Chrome azurol S dissolved in 50mL of aquadest, and mixed with 10mL of FeCl3.6H2O solutions (1mM of FeCl3.6H2O+10mM of HCl) then added by 72.9mg of HDTMA dissolved in 40mL of aquadest, the solutions then was added to 900mL King? s B agar (Hymedia©,, 10/06/2021 ÿú On chrome azurol S agar 45.1% of pathogenic E. coli. were found to produce siderophores, 12.5% isolates were found to produce catechol-type siderophore and 35.4% isolates produced hydroxamate-type.
Please contact Dr. Steven Wilhelm (wilhelm@utk.edu) for additional information regarding this protocol.Modified from Schwyn, B. & Neilands, J. B. Universal chemical assay for.
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